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KMID : 0613820090190111666
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Journal of Life Science
2009 Volume.19 No. 11 p.1666 ~ p.1671
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Characterization of the Stigma side Self-incompatibility Genes in a Self-compatible Brassica oleracea
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Park Jong-In
Lee In-Ho
Jung Gun-Ho
Nou Il-Sup
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Abstract
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In Brassica, S locus glycoprotein (SLG) and S locus receptor kinase (SRK) genes function together for self-recognition in the self-incompatibility response. In addition, a water channel called aquaporins (MOD) is required for the self-incompatibility response. In this study, we isolated the SC-SLG, SC-SRK, and SC-MOD genes from a self-compatible line of B. oleracea. In the self-compatible line, the SC-SLG, SC-SRK, and SC-MOD genes showed the highest degree of sequence similarity with published data and to normal expression by RT-PCR. Therefore, it can be concluded that the SCR/SP11 gene of the B. oleracea pollen may not function and/or that mutations may occur in genes for self-incompatibility that are not linked to the S locus region.
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KEYWORD
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Brassica oleracea, self-incompatibility, self-compatibility, S-locus glycoprotein, S-locus receptor kinase
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